Relationship of field and in vitro fertility of dairy bulls with sperm parameters, including DAG1 and SERPINA5 proteins

Introduction Sperm interacts with the female reproductive tract and oocyte through proteins, these cell-to-cell interactions may play a role in sperm fertility. For consideration of protein as potential marker fertility, there must be variability expressed among animals. The proteins dystroglycan (DAG1) plasma serine protease inhibitor (SERPINA5) have been reported to interactions. Thus, objectives this study were characterize localization abundance DAG1 SERPINA5 bovine sperm, investigate relationship field fertility (i.e., sire conception rate; SCR), vitro embryo production (IVP), parameters. Material methods Dairy bulls (n = 22) classified high-SCR (SCR > 1.0) or low-SCR < –4.0), good [blastocyst (BL)-by-cleavage (CL) ratio (BL/CL) 39%] poor (BL/CL 38%) BL/CL. was evaluated for immunolocalization, concentration two separate ejaculates. Variance between compared within using generalized linear model (GLM) procedure. SCR IVP classification on concentrations, percentage tail labeled SERPINA5, SCR, total progressive motility, membrane integrity (PMI), CL, BL, BL/CL GLIMMIX procedure, correlations variables evaluated. Results Both localized head; however, also tail. There greater variance than ( P 0.0001; 69.4 vs . 49.1, respectively) 325.8 285.4, respectively). positive correlation concentrations 0.01; r 0.54). In addition, correlated PMI 0.05; 0.44). no classifications (P ≥ 0.55), 0.54), 0.22). Discussion conclusion, head, SERPINA 5 Concentrations head each other. PMI; neither associated IVP. when by immunofluorescent microscopy, are variable not markers bull sperm.